Involvement of NO in Antinociception of NSAIDS in Murine Formalin Hind Paw Assay

Vista sencilla de ítem
dc.contributor.authorNoriega, Viviana
dc.contributor.authorMiranda, Hugo F.
dc.contributor.authorPrieto, Juan Carlos
dc.contributor.authorSotomayor-Zárate, Ramón
dc.contributor.authorSierralta, Fernando
dc.date.accessioned2022-03-08T20:19:46Z
dc.date.available2022-03-08T20:19:46Z
dc.date.issued2020
dc.description.abstractThere are different animal models to evaluate pain among them the formalin hind paw assay which is widely used since some of its events appear to be similar to the clinical pain of humans. The assay in which a dilute solution of formalin is injected into the dorsal hindpaw of a murine produces two ‘phases’ of pain behavior separated by a inactive period. The early phase (Phase I) is probably due to direct activation of nociceptors and the second phase (Phase II) is due to ongoing inflammatory input and central sensitization. Mice were used to determine the potency antinociceptive of piroxicam (1,3,10,and 30 mg/kg), parecoxib (0.3, 1,3,10 and 30 mg/kg), dexketoprofen (3,10,30 and 100 mg/kg) and ketoprofen (3,10,30 and 100 mg/kg). Doseresponse for each NSAIDs were created before and after 5 mg/kg of LNAME i.p. or 5 mg/kg i.p. of 7nitroindazole. A leastsquares linear regression analysis of the log dose–response curves allowed the calculation of the dose that produced 50 % of antinociception (ED50) for each drug. The ED50 demonstrated the following rank order of potency, in the phase I: piroxicam > dexketoprofen > ketoprofen > parecoxib and in the phase II: piroxicam > ketoprofen > parecoxib > dexketoprofen. Pretreatment of the mice with LNAME or 7nitroindazol induced a significant increase of the analgesic power of the NSAIDs, with a significant reduction of the ED50. It is suggested that NO may be involved in both phases of the trial, which means that nitric oxide regulates the bioactivity of NSAIDs.en_ES
dc.facultadFacultad de Cienciasen_ES
dc.identifier.doihttps://doi.org/10.1055/a10955418
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/3796
dc.language.isoenen_ES
dc.publisherThiemeen_ES
dc.rights© Georg Thieme Verlag KG Stuttgart · New Yorken_ES
dc.sourceDrug Researchen_ES
dc.subjectANTIINFLAMMATORYen_ES
dc.subjectDRUGSen_ES
dc.subjectPHARMACOLOGYen_ES
dc.subjectINFLAMMATIONen_ES
dc.titleInvolvement of NO in Antinociception of NSAIDS in Murine Formalin Hind Paw Assayen_ES
dc.typeArticuloen_ES
uv.catalogadorRCR DIBRAen_ES
uv.departamentoInstituto de Fisiologiaen_ES

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
2020 Sotomayor-Zárate R. Drug Research.pdf
Tamaño:
399.83 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
384 B
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Artículos investigadores UV