Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts

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dc.contributor.authorFerrero, Romina L.
dc.contributor.authorSoto-Maldonado, Carmen
dc.contributor.authorWeinstein-Oppenheimer, Caroline
dc.contributor.authorCabrera-Muñoz, Zaida
dc.contributor.authorZúñiga-Hansen, María Elvira
dc.date.accessioned2022-11-30T02:46:19Z
dc.date.available2022-11-30T02:46:19Z
dc.date.issued2021
dc.description.abstractDefatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agenten_ES
dc.facultadFacultad de Farmaciaen_ES
dc.file.nameFerrero_Ant2021.pdf
dc.identifier.citationFerrero, R.L.; Soto-Maldonado, C.; Weinstein-Oppenheimer, C.; Cabrera-Muñoz, Z.; Zúñiga-Hansen, M.E. Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts. Foods 2021, 10, 309. https://doi.org/10.3390/foods10020309en_ES
dc.identifier.doihttps://doi.org/10.3390/foods10020309
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/7319
dc.languageen
dc.publisherMDPI
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.sourceBreast Cancer Cells and Human Fibroblasts
dc.subjectBIOACTIVE PEPTIDESen_ES
dc.subjectANTIPROLIFERATIVE ACTIVITYen_ES
dc.subjectBREAST CANCERen_ES
dc.subjectANTICANCERen_ES
dc.subjectMEAL RAPESEEDen_ES
dc.titleAntiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
dc.typeArticulo
uv.departamentoEscuela de Quimica y Farmacia

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