Chemical Composition, Antioxidant and Anticancer Activities of Leptocarpha rivularis DC Flower Extracts

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dc.contributor.authorMontenegro, Jorge
dc.contributor.authorMoreira, Jorge
dc.contributor.authorRamirez, Ingrid
dc.contributor.authorDorta, Fernando
dc.contributor.authorSánchez, Elizabeth
dc.contributor.authorAlfaro, Juan Felipe
dc.contributor.authorValenzuela, Manuel
dc.contributor.authorJara-Gutiérrez, Carlos
dc.contributor.authorMuñoz, Ociel
dc.contributor.authorAlvear, Matías
dc.contributor.authorWerner, Enrique
dc.contributor.authorMadrid, Alejandro
dc.contributor.authorVillena, Joan
dc.contributor.authorSeeger, Michael
dc.date.accessioned2022-11-30T02:46:37Z
dc.date.available2022-11-30T02:46:37Z
dc.date.issued2021
dc.description.abstractAn evaluation of antioxidant and anticancer activity was screened in Leptocarpha rivularis DC flower extracts using four solvents (n-hexane (Hex), dichloromethane (DCM), ethyl acetate (AcOEt), and ethanol (EtOH)). Extracts were compared for total extract flavonoids and phenol contents, antioxidant activity (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric reducing antioxidant potential (FRAP), total reactive antioxidant properties (TRAP) and oxygen radical absorbance capacity (ORAC)) across a determined value of reduced/oxidized glutathione (GSH/GSSG), and cell viability (the sulforhodamine B (SRB) assay). The most active extracts were analyzed by chromatographic analysis (GC/MS) and tested for apoptotic pathways. Extracts from Hex, DCM and AcOEt reduced cell viability, caused changes in cell morphology, affected mitochondrial membrane permeability, and induced caspase activation in tumor cell lines HT-29, PC-3, and MCF-7. These effects were generally less pronounced in the HEK-293 cell line (nontumor cells), indicating clear selectivity towards tumor cell lines. We attribute likely extract activity to the presence of sesquiterpene lactones, in combination with other components like steroids and flavonoids.en_ES
dc.facultadFacultad de Medicinaen_ES
dc.file.nameMontenegro_Chem2021.pdf
dc.identifier.citationMontenegro, I.; Moreira, J.; Ramirez, I.; Dorta, F.; Sanchez, E.;Alfaro, J.F.; Valenzuela, M.;Jara-Gutiérrez, C.; Muñoz, O.;Alvear, M.; et al. Chemical Composition, Antioxidant and Anticancer Activities of Leptocarpha rivularis DC Flower Extracts. Molecules 2021, 26, 67. https://doi.org/10.3390/molecules26010067en_ES
dc.identifier.doihttps://doi.org/10.3390/mole-cules26010067
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/7442
dc.languageen
dc.publisherMDPI
dc.rightsCopyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.sourceMolecules
dc.subjectPALO NEGROen_ES
dc.subjectANTIOXIDANTen_ES
dc.subjectCANCER CELL LINEen_ES
dc.titleChemical Composition, Antioxidant and Anticancer Activities of Leptocarpha rivularis DC Flower Extracts
dc.typeArticulo
uv.departamentoEscuela de Obstetricia y Puericultura

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