Examinando por Autor "Weinstein-Oppenheimer, Caroline"

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    Alfabetización estadística y comunicación de riesgo para la vacunación contra la COVID-19: una revisión de alcance
    (Organización Panamericana De La Salud, 2021) Palmeiro-Silva, Yasna K.; Weinstein-Oppenheimer, Caroline; Henríquez-Roldán, Carlos Felipe; Bangdiwala, Shrikant I.
    Objetivo. Describir el papel que desempeñan la alfabetización estadística y la correcta comunicación de riesgo en las estrategias de comunicación relacionadas con la vacunación contra la COVID-19. Métodos. Se realizó una revisión de alcance en enero del 2021, con las palabras clave “ statistical literacy ”, “ risk communication”, “health communication ” y “ pandemic ” en las bases de datos de la Biblioteca Virtual en Salud de la Organización Panamericana de la Salud, PubMed, Web of Science, EBSCO y Google Académico. No se aplicaron filtros para fechas, idioma o tipos de publicación. Resultados. De los 87 artículos identificados, cuatro cumplieron con los criterios de inclusión. Se recono-cieron cuatro mensajes principales que relacionan la alfabetización estadística y la comunicación de riesgo: 1) la comunicación de riesgo y el nivel de alfabetización estadística afectan a la toma de decisión individual y colectiva, 2) la comunicación de la incertidumbre debe incluir lo que se sabe y no se sabe respecto a las estadísticas y los riesgos, 3) el uso de gráficos y visualizaciones es clave para informar apropiadamente a la población y 4) deben utilizarse diferentes formatos para mejorar la comunicación, siempre ajustados al nivel de alfabetización estadística de la población. Conclusiones. La alfabetización estadística desempeña un papel clave en la comunicación de los riesgos relacionados con la salud en general y la vacunación contra la COVID-19 en particular. En situaciones de emergencia sanitaria, la correcta comunicación de riesgo y de la incertidumbre asociada debe ser clara, transparente y oportuna.
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    Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
    (MDPI, 2021) Ferrero, Romina L.; Soto-Maldonado, Carmen; Weinstein-Oppenheimer, Caroline; Cabrera-Muñoz, Zaida; Zúñiga-Hansen, María Elvira
    Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agent
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    Inhibition of Caco-2 and MCF-7 cancer cells using chalcones: synthesis, biological evaluation and computational study
    (Taylor & Francis, 2021) Mellado, Marco; Reyna-Jeldes, Mauricio; Weinstein-Oppenheimer, Caroline; Coddou, Claudio; Jara-Gutierrez, Carlos; Villena, Joan; Aguilar, Luis F.
    Cancer is the second death cause worldwide, with breast and colon cancer among the most prevalent types. Traditional treatment strategies have several side effects that inspire the development of novel anticancer agents derived from natural sources, like chalcone derivatives. For this investigation, twenty-three chalcones (4a-w) were synthesized and evaluated as antiproliferative agents against MCF-7 and Caco-2 cells, finding three and two compounds with similar or higher antiproliferative activity than daunorubicin, while only two chalcones showed better selectivity indexes than daunorubicin on MCF-7. From these results, we developed good-performance QSAR models (r> 0.850, q2 >0.650), finding several structural features that could modify chalcone activity and selectivity. According to these models, chalcones 4w and 4t have high potency and selectivity against Caco-2 and MCF-7, respectively, which make them attractive candidates for hit-to-lead development of ROS-independent pro apoptotic agents.
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    Natural isoflavonoids in invasive cancer therapy: From bench to bedside
    (Wiley, 2021) Cayetano-Salazar, Lorena; Olea-Flores, Monserrat; Zuñiga-Eulogio, Miriam D.; Weinstein-Oppenheimer, Caroline; Fernández-Tilapa, Gloria; Mendoza-Catalán, Miguel A.; Zacapala-Gómez, Ana E.; Ortiz-Ortiz, Julio; Ortuño-Pineda, Carlos; Navarro-Tito, Napoleón
    Cancer is a public health problem worldwide, and one of the crucial steps within tumor progression is the invasion and metastasis of cancer cells, which are directly related to cancer-associated deaths in patients. Recognizing the molecular markers involved in invasion and metastasis is essential to find targeted therapies in cancer. Interestingly, about 50% of the discovered drugs used in chemotherapy have been obtained from natural sources such as plants, including isoflavonoids. Until now, most drugs are used in chemotherapy targeting proliferation and apoptosis-related molecules. Here, we review recent studies about the effect of isoflavonoids on molecular targets and signaling pathways related to invasion and metastasis in cancer cell cultures, in vivo assays, and clinical trials. This review also reports that glycitein, daidzein, and genistein are the isoflavonoids most studied in preclinical and clinical trials and displayed the most anticancer activity targeting invasion-related proteins such as MMP-2 and MMP-9 and also EMT-associated proteins. Therefore, the diversity of isoflavonoids is promising molecules to be used as chemotherapeutic in invasive cancer. In the future, more clinical trials are needed to validate the effectiveness of the various natural isoflavonoids in the treatment of invasive cancer.
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    Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling
    (MDPI, 2021) Flores-Muñoz, Carolina; Maripillán, Jaime; Vásquez-Navarrete, Jacqueline; Novoa-Molina, Joel; Ceriani, Ricardo; Sánchez, Helmuth A.; Abbott, Ana C.; Weinstein-Oppenheimer, Caroline; Brown, Donald I.; Cárdenas, Ana María; García, Isaac E.; Martínez , Agustín D.
    Wound healing is a dynamic process required to maintain skin integrity and which relies on the precise migration of different cell types. A key molecule that regulates this process is ATP. However, the mechanisms involved in extracellular ATP management are poorly understood, particularly in the human dermis. Here, we explore the role, in human fibroblast migration during wound healing, of Pannexin 1 channels and their relationship with purinergic signals and in vivo cell surface filamentous actin dynamics. Using siRNA against Panx isoforms and different Panx1 channel inhibitors, we demonstrate in cultured human dermal fibroblasts that the absence or inhibition of Panx1 channels accelerates cell migration, increases single-cell motility, and promotes actin redistribution. These changes occur through a mechanism that involves the release of ATP to the extracellular space through a Panx1-dependent mechanism and the activation of the purinergic receptor P2X7. Together, these findings point to a pivotal role of Panx1 channels in skin fibroblast migration and suggest that these channels could be a useful pharmacological target to promote damaged skin healing.