Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes
Archivos
Fecha
2021
Profesor Guía
Formato del documento
Articulo
ORCID Autor
Título de la revista
ISSN de la revista
Título del volumen
Editor
Elsevier
Ubicación
ISBN
ISSN
item.page.issne
item.page.doiurl
Facultad
Facultad de Medicina
Departamento o Escuela
Centro de Investigaciones Biomedicas
Determinador
Recolector
Especie
Nota general
Resumen
In fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish cell models have focused on ROS production and the respiratory burst mechanism. However, the effects on enzymatic antioxidant defense on salmon leukocytes have not been evaluated. This study aims to evaluate the enzymatic antioxidant defense and ROS-induced cell damage in Salmon Head Kidney-1 (SHK-1) cell line exposed to polyphenol-enriched extract from Sambucus nigra flowers. Results: Firstly, the Total Reactive Antioxidant Power (TRAP) assay of elderflower polyphenol (EP) was evaluated, showing 459 and 489 times more active than gallic acid and butyl hydroxy toluene (BHT), respectively. The toxic effect of EP on salmon cells was not significant at concentrations below 120 mg/mL and no hemolysis activity was observed between 20 and 400 mg/mL. The treatment of SHK-1 cell line with EP decreased both the lipid peroxidation and protein oxidation induced by H2O2, which could be associated with decreasing oxidative stress in the SHK-1 cells since the GSH/GSSG ratio increased when only EP was added. Conclusions: These results suggest that plant extracts enriched with polyphenols could improve the enzymatic antioxidant defense of salmon leukocytes and protect the cells against ROS-induced cell damage.
Descripción
Lugar de Publicación
Auspiciador
Palabras clave
ATLANTIC SALMON (SALMO SALAR), ELDERFLOWER POLYPHENOL, ENZYMATIC ANTIOXIDANT DEFENSE, FISH AQUACULTURE, FISH FARMING, OXIDATIVE STRESS, PATHOGEN RESISTANCE, PLANT EXTRACTS, RAINBOW TROUT (ONCORHYNCHUS MYKISS), ROS-INDUCED CELL DAMAGE, SALMON LEUKOCYTE
Licencia
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).