Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes

Vista sencilla de ítem
dc.contributor.authorSantana, Paula Andrea
dc.contributor.authorJara-Gutiérrez, Carlos
dc.contributor.authorMellado, Marco
dc.contributor.authorForero, Juan Carlos
dc.contributor.authorGuzmán, Fanny
dc.contributor.authorBarriga, Andrés
dc.contributor.authorAlbericio, Fernando
dc.contributor.authorÁlvarez, Claudio Andrés
dc.date.accessioned2022-11-30T02:46:26Z
dc.date.available2022-11-30T02:46:26Z
dc.date.issued2021
dc.description.abstractIn fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish cell models have focused on ROS production and the respiratory burst mechanism. However, the effects on enzymatic antioxidant defense on salmon leukocytes have not been evaluated. This study aims to evaluate the enzymatic antioxidant defense and ROS-induced cell damage in Salmon Head Kidney-1 (SHK-1) cell line exposed to polyphenol-enriched extract from Sambucus nigra flowers. Results: Firstly, the Total Reactive Antioxidant Power (TRAP) assay of elderflower polyphenol (EP) was evaluated, showing 459 and 489 times more active than gallic acid and butyl hydroxy toluene (BHT), respectively. The toxic effect of EP on salmon cells was not significant at concentrations below 120 mg/mL and no hemolysis activity was observed between 20 and 400 mg/mL. The treatment of SHK-1 cell line with EP decreased both the lipid peroxidation and protein oxidation induced by H2O2, which could be associated with decreasing oxidative stress in the SHK-1 cells since the GSH/GSSG ratio increased when only EP was added. Conclusions: These results suggest that plant extracts enriched with polyphenols could improve the enzymatic antioxidant defense of salmon leukocytes and protect the cells against ROS-induced cell damage.en_ES
dc.facultadFacultad de Medicinaen_ES
dc.file.nameJara_Effe2021.pdf
dc.identifier.citationSantana PA, Jara-Gutiérrez C, Mellado M, et al. Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.004en_ES
dc.identifier.doihttps://doi.org/10.1016/j.ejbt.2021.04.004
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/7375
dc.languageen
dc.publisherElsevier
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.sourceElectronic Journal of Biotechnology
dc.subjectATLANTIC SALMON (SALMO SALAR)en_ES
dc.subjectELDERFLOWER POLYPHENOLen_ES
dc.subjectENZYMATIC ANTIOXIDANT DEFENSEen_ES
dc.subjectFISH AQUACULTUREen_ES
dc.subjectFISH FARMINGen_ES
dc.subjectOXIDATIVE STRESSen_ES
dc.subjectPATHOGEN RESISTANCEen_ES
dc.subjectPLANT EXTRACTSen_ES
dc.subjectRAINBOW TROUT (ONCORHYNCHUS MYKISS)en_ES
dc.subjectROS-INDUCED CELL DAMAGEen_ES
dc.subjectSALMON LEUKOCYTEen_ES
dc.titleEffects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes
dc.typeArticulo
uv.departamentoCentro de Investigaciones Biomedicas

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Jara_Effe2021.pdf
Tamaño:
836.65 KB
Formato:
Adobe Portable Document Format
Descargar

Colecciones

Artículos investigadores UV