Protective effect of the hydroalcoholic extract from Lampaya medicinalis Phil. (Verbenaceae) on palmitic acid- impaired insulin signaling in 3T3-L1 adipocytes

Vista sencilla de ítem
dc.contributor.authorOrmazabal, Paulina
dc.contributor.authorHerrera, Karin
dc.contributor.authorCifuentes, Mariana
dc.contributor.authorParedes, Adrián
dc.contributor.authorMorales, Glauco
dc.contributor.authorCruz, Gonzalo
dc.date.accessioned2022-03-17T20:06:29Z
dc.date.available2022-03-17T20:06:29Z
dc.date.issued2020
dc.description.abstractBackground: Obesity is strongly associated with insulin resistance (IR). IR at the molecular level may bedefined as a diminished activation of insulin signaling-related molecules (IRS-1/Akt/AS160) as well asreduced glucose uptake. Subject with obesity have elevated plasma levels of saturated fatty acids, suchas palmitic acid (PA), which triggers insulin signaling disruption in vivo and in vitro. Infusions of Lampayamedicinalis Phil. (Verbenaceae) are used in folk medicine of Northern Chile to counteract inflammatorydiseases. Hydroethanolic extracts of lampaya (HEL) contain considerable amounts of flavonoids that mayexplain the biological activity of the plant. The aim of this study was to assess whether HEL exposureprotects against PA-disrupted insulin signaling and glucose uptake in adipocytes.Methods: Cytotoxicity of a range of HEL concentrations (0.01–10 g/mL) was evaluated in 3T3-L1adipocytes. Cells were exposed or not to 0.1 g/mL of HEL before adding 0.65 mM PA or vehicle andincubated with 100 nM insulin (or vehicle) for 15 min. Phosphorylation of Tyr-IRS-1, Ser-Akt, Thr-AS160was evaluated by Western blot. Glucose uptake was assessed using the 2-NBDG analogue.Results: HEL was not cytotoxic at any concentration assessed. PA-induced reduction in insulin-stimulatedphosphorylation of IRS-1, Akt and AS160 and glucose uptake were abolished by co-treatment with HEL.Conclusion: These findings give new insights about the effect of HEL ameliorating PA- impaired IRS-1/Akt/AS160 pathway and glucose uptake in adipocytes. More studies should focus on lampaya, sincemight represent a preventive approach in individuals whose circulating PA levels contribute to IR.en_ES
dc.facultadFacultad de Cienciasen_ES
dc.identifier.doihttps://doi.org/10.1016/j.orcp.2020.11.001
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/3876
dc.language.isoenen_ES
dc.publisherElsevieren_ES
dc.rights© 2020 Asia Oceania Association for the Study of Obesity. Published by Elsevier Ltd. All rights reserved.en_ES
dc.sourceObesity Research & Clinical Practiceen_ES
dc.subjectADIPOCYTEen_ES
dc.subjectPALMITIC ACIDen_ES
dc.subjectINSULIN SIGNALINGen_ES
dc.subjectGLUCOSE UPTAKEen_ES
dc.subjectLAMPAYA MEDICINALISAen_ES
dc.titleProtective effect of the hydroalcoholic extract from Lampaya medicinalis Phil. (Verbenaceae) on palmitic acid- impaired insulin signaling in 3T3-L1 adipocytesen_ES
dc.typeArticuloen_ES
uv.catalogadorRCR DIBRAen_ES
uv.departamentoInstituto de Fisiologiaen_ES

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
1-s2.0-S1871403X20306049-main.pdf
Tamaño:
1.22 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
384 B
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Artículos investigadores UV