The FGF2-induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic-dependent pathway

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dc.contributor.authorRecabal, Antonia
dc.contributor.authorFernández, Paola
dc.contributor.authorLópez, Sergio
dc.contributor.authorBarahona, María J.
dc.contributor.authorOrdenes, Patricio
dc.contributor.authorPalma, Alejandra
dc.contributor.authorElizondo-Vega, Roberto
dc.contributor.authorFarkas, Carlos
dc.contributor.authorUribe, Amparo
dc.contributor.authorCaprile, Teresa
dc.contributor.authorSáez, Juan C.
dc.contributor.authorGarcía-Robles, María A.
dc.date.accessioned2022-11-30T02:46:50Z
dc.date.available2022-11-30T02:46:50Z
dc.date.issued2021
dc.description.abstractIn the adult hypothalamus, the neuronal precursor role is attributed to the radial glia-like cells that line the third-ventricle (3V) wall called tanycytes. Under nutritional cues, including hypercaloric diets, tanycytes proliferate and differentiate into mature neurons that moderate body weight, suggesting that hypothalamic neurogenesis is an adaptive mechanism in response to metabolic changes. Previous studies have shown that the tanycyte glucosensing mechanism depends on connexin-43 hemichannels (Cx43 HCs), purine release, and increased intracellular free calcium ion concentration [(Ca2+)i] mediated by purinergic P2Y receptors. Since, Fibroblast Growth Factor 2 (FGF2) causes similar purinergic events in other cell types, we hypothesize that this pathway can be also activated by FGF2 in tanycytes to promote their proliferation. Here, we used bromodeoxyuridine (BrdU) incorporation to evaluate if FGF2-induced tanycyte cell division is sensitive to Cx43 HC inhibition in vitro and in vivo. Immunocytochemical analyses showed that cultured tanycytes maintain the expression of in situ markers. After FGF2 exposure, tanycytic Cx43 HCs opened, enabling release of ATP to the extracellular milieu. Moreover, application of external ATP was enough to induce their cell division, which could be suppressed by Cx43 HC or P2Y1-receptor inhibitors. Similarly, in vivo experiments performed on rats by continuous infusion of FGF2 and a Cx43 HC inhibitor into the 3V, demonstrated that FGF2-induced β-tanycyte proliferation is sensitive to Cx43 HC blockade. Thus, FGF2 induced Cx43 HC opening, triggered purinergic signaling, and increased β-tanycytes proliferation, highlighting some of the molecular mechanisms involved in the cell division response of tanycyte.en_ES
dc.facultadFacultad de Cienciasen_ES
dc.file.nameRecabal_Fgf2021.pdf
dc.identifier.doihttps://doi.org/10.1111/jnc.15188
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/7508
dc.languageen
dc.publisherWiley
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.sourceJournal of Neurochemistry
dc.subjectCELL BIOLOGYen_ES
dc.subjectGLIOGENESISen_ES
dc.subjectPURINERGIC RECEPTORSen_ES
dc.subjectSIGNAL TRANSDUCTIONSen_ES
dc.subjectTANYCYTEen_ES
dc.titleThe FGF2-induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic-dependent pathway
dc.typeArticulo
uv.departamentoCentro Interdisciplinario de Neurociencia de Valparaiso

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