Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling

Vista sencilla de ítem
dc.contributor.authorFlores-Muñoz, Carolina
dc.contributor.authorMaripillán, Jaime
dc.contributor.authorVásquez-Navarrete, Jacqueline
dc.contributor.authorNovoa-Molina, Joel
dc.contributor.authorCeriani, Ricardo
dc.contributor.authorSánchez, Helmuth A.
dc.contributor.authorAbbott, Ana C.
dc.contributor.authorWeinstein-Oppenheimer, Caroline
dc.contributor.authorBrown, Donald I.
dc.contributor.authorCárdenas, Ana María
dc.contributor.authorGarcía, Isaac E.
dc.contributor.authorMartínez , Agustín D.
dc.date.accessioned2022-11-30T02:46:20Z
dc.date.available2022-11-30T02:46:20Z
dc.date.issued2021
dc.description.abstractWound healing is a dynamic process required to maintain skin integrity and which relies on the precise migration of different cell types. A key molecule that regulates this process is ATP. However, the mechanisms involved in extracellular ATP management are poorly understood, particularly in the human dermis. Here, we explore the role, in human fibroblast migration during wound healing, of Pannexin 1 channels and their relationship with purinergic signals and in vivo cell surface filamentous actin dynamics. Using siRNA against Panx isoforms and different Panx1 channel inhibitors, we demonstrate in cultured human dermal fibroblasts that the absence or inhibition of Panx1 channels accelerates cell migration, increases single-cell motility, and promotes actin redistribution. These changes occur through a mechanism that involves the release of ATP to the extracellular space through a Panx1-dependent mechanism and the activation of the purinergic receptor P2X7. Together, these findings point to a pivotal role of Panx1 channels in skin fibroblast migration and suggest that these channels could be a useful pharmacological target to promote damaged skin healing.en_ES
dc.facultadFacultad de Cienciasen_ES
dc.file.nameFlores_Res2021.pdf
dc.identifier.citationFlores-Muñoz, C.; Maripillán, J.; Vásquez-Navarrete, J.; Novoa-Molina, J.; Ceriani, R.; Sánchez, H.A.; Abbott, A.C.; Weinstein-Oppenheimer, C.; Brown, D.I.; Cárdenas, A.M.; García, I.E.; Martínez, A.D. Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling. Int. J. Mol. Sci. 2021, 22, 1069. https://doi.org/10.3390/ijms22031069en_ES
dc.identifier.doihttps://doi.org/10.3390/ijms22031069
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/7327
dc.languageen
dc.publisherMDPI
dc.rights© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.sourceInternational Journal of Molecular Sciences
dc.subjectPANNEXIN 1en_ES
dc.subjectACTIN CYTOSKELETONen_ES
dc.subjectCELL MIGRATIONen_ES
dc.subjectWOUND HEALINGen_ES
dc.subjectHUMAN DERMAL FIBROBLASTSen_ES
dc.subjectPURINERGIC RECEPTORen_ES
dc.titleRestraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling
dc.typeArticulo
uv.departamentoCentro Interdisciplinario de Neurociencia de Valparaiso

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Flores_Res2021.pdf
Tamaño:
3.16 MB
Formato:
Adobe Portable Document Format
Descargar

Colecciones

Artículos investigadores UV