Examinando por Autor "Arriagada, Javiera"

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    Ítem
    Anti-parasitic drugs modulate the non-selective channels formed by connexins or pannexins
    (Elsevier, 2021) Güiza, Juan; Arriagada, Javiera; Rodríguez, Luis; Gutiérrez, Camila; Duarte, Yorley; Sáez, Juan C.; Vega, José L.
    The proteins connexins, innexins, and pannexins are the subunits of non-selective channels present in the cell membrane in vertebrates (connexins and pannexins) and invertebrates (innexins). These channels allow the transfer of ions and molecules across the cell membrane or, and in many cases, between the cytoplasm of neighboring cells. These channels participate in various physiological processes, particularly under pathophysiological conditions, such as bacterial, viral, and parasitic infections. Interestingly, some anti-parasitic drugs also block connexin- or pannexin-formed channels. Their effects on host channels permeable to molecules that favor parasitic infection can further explain the anti-parasitic effects of some of these compounds. In this review, the effects of drugs with known anti-parasitic activity that modulate non-selective channels formed by connexins or pannexins are discussed. Previous studies that have reported the presence of these proteins in worms, ectoparasites, and protozoa that cause parasitic infections have also been reviewed.
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    Ítem
    Aplicación del proceso de alta por el profesional de enfermería en usuarios post quirúrgicos de un servicio de cirugía
    (Universidad de Valparaíso, 2006) Arriagada, Javiera; Bravo, María Antonieta; Carrasco, Carolina; Jaman, Paula; Serra, María Luisa; Lazcano, María Raquel
    El objetivo de este estudio es evaluar la aplicación del Proceso de Alta, que realiza el Profesional de Enfermería, para promover la continuidad de los cuidados en el usuario post quirúrgico. El fin de este Proceso es proporcionar a los usuarios y a las familias el conocimiento y las técnicas precisas para cubrir las necesidades de asistencia sanitaria en el hogar y favorecer el autocuidado. Consta de tres etapas: Ingreso Hospitalario, Hospitalización y Egreso Hospitalario. Cada una de estas etapas fue medida en cien Procesos de Alta realizados en usuarios adultos post quirúrgicos en el Hospital Dr. Gustavo Fricke de Viña del Mar.
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    Ítem
    Unnexins: Homologs of innexin proteins in Trypanosomatidae parasites
    (Wiley, 2022) Güiza, Juan; García, Aníbal; Arriagada, Javiera; Gutiérrez, Camila; González, Jorge; Márquez-Miranda, Valeria; Alegría-Arcos, Melissa; Duarte, Yorley; Rojas, Maximiliano; González-Nilo, Fernando; Sáez, Juan C.; Vega, José L.
    Large-pore channels, including those formed by connexin, pannexin, innexin proteins, are part of a broad family of plasma membrane channels found in vertebrates and invertebrates, which share topology features. Despite their relevance in parasitic diseases such as Chagas and malaria, it was unknown whether these large-pore channels are present in unicellular organisms. We identified 14 putative proteins in Trypanosomatidae parasites as presumptive homologs of innexin proteins. All proteins possess the canonical motif of the innexin family, a pentapeptide YYQWV, and 10 of them share a classical membrane topology of large-pore channels. A sequence similarity network analysis confirmed their closeness to innexin proteins. A bioinformatic model showed that a homolog of Trypanosoma cruzi (T. cruzi) could presumptively form a stable octamer channel with a highly positive electrostatic potential in the internal cavities and extracellular entrance due to the notable predominance of residues such as Arg or Lys. In vitro dye uptake assays showed that divalent cations-free solution increases YO-PRO-1 uptake and hyperosmotic stress increases DAPI uptake in epimastigotes of T. cruzi. Those effects were sensitive to probenecid. Furthermore, probenecid reduced the proliferation and transformation of T. cruzi. Moreover, probenecid or carbenoxolone increased the parasite sensitivity to antiparasitic drugs commonly used in therapy against Chagas. Our study suggests the existence of innexin homologs in unicellular organisms, which could be protein subunits of new large-pore channels in unicellular organisms.