Genomic plasticity in the olfactory epithelium mediated by odorant exposure in zebrafish (Danio rerio)

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dc.contributor.advisorWhitlock, Kathleen (Directora de tesis)
dc.contributor.authorCalfún Medina, Cristian
dc.date.accessioned2022-08-04T19:53:58Z
dc.date.available2022-08-04T19:53:58Z
dc.date.issued2017-08-25
dc.description.abstractWe have previously shown that exposure to phenyl ethyl alcohol (PEA) causes an increase in the expression of the transcription factor otx2 in the olfactory epithelium (OE) of juvenile zebrafish, and this change is correlated with the formation of an odor memory of PEA. Here, we show that the changes in otx2 expression are specific to βPEA: exposure to αPEA did not affect otx2 expression. We identified 34 olfactory receptors (ORs) representing 16 families on 4 different chromosomes as candidates for direct regulation of OR expression via Otx2. Subsequent in silico analysis uncovered Hnf3b binding sites closely associated with Otx2 binding sites in the regions flanking the ORs. Analysis by quantitative polymerase chain reaction and RNA-seq of OR expression in developing zebrafish exposed to different isomers of PEA showed that a subset of ORs containing both Otx2/Hnf3b binding sites were down regulated only in βPEA-exposed juveniles and this change persisted through adult life. Localization of OR expression by in situ hybridization indicates the down regulation occurs at the level of RNA and not the number of cells expressing a given receptor. Finally, analysis of immediate early gene expression in the OE did not reveal changes in c-fos expression in response to either αPEA or βPEA.en_ES
dc.description.sponsorshipFONDECYT-1111046, Proyecto Instituto Milenio PO9-022-Fen_ES
dc.facultadFacultad de Cienciasen_ES
dc.identifier.citationCalfún, C. (2017). Genomic plasticity in the olfactory epithelium mediated by odorant exposure in zebrafish (Danio rerio) (Tesis de postgrado). Universidad de Valparaíso, Valparaíso, Chile.en_ES
dc.identifier.urihttp://repositoriobibliotecas.uv.cl/handle/uvscl/4706
dc.language.isoenen_ES
dc.publisherUniversidad de Valparaísoen_ES
dc.subjectMICROBIOLOGIAen_ES
dc.subjectNEUROCIENCIASen_ES
dc.subjectOLFATOen_ES
dc.titleGenomic plasticity in the olfactory epithelium mediated by odorant exposure in zebrafish (Danio rerio)en_ES
dc.typeTesisen_ES
uv.catalogadorPJR CIENen_ES
uv.departamentoInstituto de Neurocienciaen_ES
uv.notageneralDoctor en Ciencias con Mención en Neurocienciaen_ES

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