Genomic plasticity in the olfactory epithelium mediated by odorant exposure in zebrafish (Danio rerio)
Fecha
2017-08-25
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Universidad de Valparaíso
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Facultad
Facultad de Ciencias
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Instituto de Neurociencia
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Nota general
Doctor en Ciencias con Mención en Neurociencia
Resumen
We have previously shown that exposure to phenyl ethyl alcohol (PEA) causes an increase in the
expression of the transcription factor otx2 in the olfactory epithelium (OE) of juvenile zebrafish,
and this change is correlated with the formation of an odor memory of PEA. Here, we show that
the changes in otx2 expression are specific to βPEA: exposure to αPEA did not affect otx2
expression. We identified 34 olfactory receptors (ORs) representing 16 families on 4 different
chromosomes as candidates for direct regulation of OR expression via Otx2. Subsequent in silico
analysis uncovered Hnf3b binding sites closely associated with Otx2 binding sites in the regions
flanking the ORs. Analysis by quantitative polymerase chain reaction and RNA-seq of OR
expression in developing zebrafish exposed to different isomers of PEA showed that a subset of
ORs containing both Otx2/Hnf3b binding sites were down regulated only in βPEA-exposed
juveniles and this change persisted through adult life. Localization of OR expression by in situ
hybridization indicates the down regulation occurs at the level of RNA and not the number of
cells expressing a given receptor. Finally, analysis of immediate early gene expression in the OE
did not reveal changes in c-fos expression in response to either αPEA or βPEA.
Descripción
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Auspiciador
FONDECYT-1111046,
Proyecto Instituto Milenio PO9-022-F
Palabras clave
MICROBIOLOGIA, NEUROCIENCIAS, OLFATO