GnRH and testosterone treatment generate GnRH neurons in the hypothalamus of adult zebrafish (Danio rerio)
Fecha
2019-07
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Universidad de Valparaíso
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Facultad
Facultad de Ciencias
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Instituto de Neurociencia
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Doctor en Ciencias con Mención en Neurociencia
Resumen
Gonadotropin-releasing hormone (GnRH) is an essential in the control of
reproduction of vertebrates. A decrease in the secretion of GnRH causes reproductive
disorders called congenital idiopathic hypogonadotropic hypogonadism (IHH), which is
characterized by absence of puberty. Once diagnosed, IHH patients can be treated with
reproductive hormone therapy to restore the disease. Interestingly, studies have
documented a reversion of the disease after discontinuation of hormone treatment,
which improves the reproductive capacities. We suggest the hormone treatment
stimulate the neurogenesis of GnRH neurons in the hypothalamus in such a way to
produce reversion in IHH patients. Here, we characterized the neurogenesis in the
preoptic area (POA) of adult zebrafish. Our results showed that GnRH and not
testosterone treatment, significantly increased the neurogenesis in the POA. In addition,
results obtained by in situ hybridization to gnrh3 and MALDI TOF, did not detect any
GnRH isoform in the hypothalamus. Therefore, our results and recent publications show
that GnRH does not affect the control of reproduction in zebrafish. In order to find a
potential candidate to replace GnRH, we cloned and characterized phoenixin/smim20
expression, which has a reproductive role in mammals. phoenixin/smim20 is widely
expressed in the brain especially in the hypothalamus of adult zebrafish.
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NEUROGENESIS, HIPOGONADISMO, GONADOTROPINA, BROTES DE ENFERMEDADES, NEUROGENESIS